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| Registros recuperados: 69 | |
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| Damm, U.; Crous, P.W.; Fourie, P.H.. |
| During a survey of Prunus wood from South Africa, isolations were made of three presumably Calosphaerialean fungi that formed hyphomycetous, phialidic anamorphs in culture. In order to reveal the phylogenetic relationship of these fungi, they were characterised on a morphological and molecular (LSU and ITS rDNA) basis. Two isolates that formed a teleomorph in culture are newly described as Calosphaeria africana sp. nov. Although asci of Calosphaeria are characterised by having non-amyloid apical rings, two functional wall layers were observed in asci of C. africana, which has hitherto not been observed in any member of the Calosphaeriaceae. However, Calosphaeriaceae (Calosphaeriales, Sordariomycetes) are not closely related to other bitunicate fungi like... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Apical ring; Ascospore discharge; Ascus dehiscence; Beta-tubulin; Calosphaeria; Calosphaeriaceae; ITS; Jattaea; LSU; Phialophora; Prunus; SSU; Systematics. |
| Ano: 2008 |
URL: http://www.repository.naturalis.nl/record/531626 |
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| Olariaga, I.; Vooren, N. van; Carbone, M.; Hansen, K.. |
| The easily recognised genus Otidea is subjected to numerous problems in species identification. A number of old names have undergone various interpretations, materials from different continents have not been compared and misidentifications occur commonly. In this context, Otidea is monographed, based on our multiple gene phylogenies assessing species boundaries and comparative morphological characters (see Hansen & Olariaga 2015). All names combined in or synonymised with Otidea are dealt with. Thirty-three species are treated, with full descriptions and colour illustrations provided for 25 of these. Five new species are described, viz. O. borealis, O. brunneoparva, O. oregonensis, O. pseudoleporina and O. subformicarum. Otidea cantharella var. minor... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Flavoscypha; ITS; ITS1 minisatellites; LSU; Otideopsis; Resinous exudates. |
| Ano: 2015 |
URL: http://www.repository.naturalis.nl/record/588730 |
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| Abdollahzadeh, J.; Javadi, A.; Zare, R.; Phillips, A.J.L.. |
| Dothiorella and Spencermartinsia are two botryosphaeriaceous genera with dark 2-celled conidia and found in parasitic, saprophytic or endophytic association with various woody host plants. Based on ITS and EF1-α sequence data and morphology, eight new species are described from Iran, New Zealand, Portugal and Spain. Of these, five species are placed in Dothiorella, namely D. iranica, D. parva, D. prunicola, D. sempervirentis and D. striata, and three species belong to Spencermartinsia named as S. citricola, S. mangiferae and S. plurivora. An identification key to the species of each genus is provided. |
| Tipo: Article / Letter to the editor |
Palavras-chave: Botryosphaeriaceae; Dothiorella; ITS; Phylogeny; Spencermartinsia; Systematics; Taxonomy. |
| Ano: 2014 |
URL: http://www.repository.naturalis.nl/record/532314 |
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| Crous, P.W.; Summerell, B.A.; Shivas, R.G.; Carnegie, A.J.; Groenewald, J.Z.. |
| Harknessiaceae is introduced as a new family in the ascomycete order Diaporthales to accommodate species of Harknessia with their Wuestneia-like teleomorphs. The family is distinguished by having pycnidial conidiomata with brown, furfuraceous margins, brown conidia with hyaline, tube-like basal appendages, longitudinal striations, and rhexolytic secession. Six species occurring on Eucalyptus are newly introduced, namely H. australiensis, H. ellipsoidea, H. pseudohawaiiensis, and H. ravenstreetina from Australia, H. kleinzeeina from South Africa, and H. viterboensis from Italy. Epitypes are designated for H. spermatoidea and H. weresubiae, both also occurring on Eucalyptus. Members of Harknessia are commonly associated with leaf spots, but also occur as... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Biodiversity; Fungal pathogens; Harknessiaceae; ITS; LSU; Phylogeny; Systematics. |
| Ano: 2012 |
URL: http://www.repository.naturalis.nl/record/532250 |
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| Rosa, Daniel Dias; UNESP; Marque, Janaina Marianno de; UNESP; Fenille, Roseli Chela; Laboratorio de Apoio Vegetal - MAPA; Souza, Nilton Luiz de; UNESP; Kuramae, Eiko Eurya; Fungal Biodiversity Center. |
| Análise filogenética através de características morfológicas e culturais é considerada inconsistente e de difícil aceitação cientiífica, uma vez que não há como estabelecer a direção da evolução de caracteres morfológicos. Os mMarcadores moleculares são mais apropriados para análises filogenéticas. O seqüeênciamento das regiões ITS1 e ITS2 (Internal Transcribed Spacer) e o gene 5.8S rDNA forami utilizados para estimar a variação e a distância genética entre 10 isolados de Phytophthora capsici. A quantidade de variação genética entre os isolados de P. capsici de diferentes regiões do estadoEstado de São Paulo foi de 0,1% a 1,6% e de 0,1% a 1,1% nas regiões ITS1 e ITS2, respectivamente, e uma distância filogenética de 78,5% entre os isolados de P. capsici e... |
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Palavras-chave: 2.01.00.00-0 Biologia Geral DNA ribossomal; ITS; FFilogenia; Phytophthora sp 2.01.00.00-0 Biologia Geral. |
| Ano: 2003 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2033 |
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| Abdollahzadeh, J.; Mohammadi Goltapeh, E.; Javadi, A.; Shams-bakhsh, M.; Zare, R.; Phillips:, A.J.L.. |
| Species in the Botryosphaeriaceae are well known as pathogens and saprobes of woody hosts, but little is known about the species that occur in Iran. In a recent survey of this family in Iran two fungi with diplodia-like anamorphs were isolated from various tree hosts. These two fungi were fully characterised in terms of morphology of the anamorphs in culture, and sequences of the ITS1/ITS2 regions of the ribosomal DNA operon and partial sequences of the translation elongation factor 1-α. Phylogenetic analyses placed them within a clade consisting of Barriopsis and Phaeobotryon species, but they were clearly distinct from known species in these genera. Therefore, they are described here as two new species, namely Barriopsis iraniana on Citrus, Mangifera and... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Citrus; EF 1-α; ITS; Mangifera; Olea; Phylogeny; Systematics; Taxonomy. |
| Ano: 2009 |
URL: http://www.repository.naturalis.nl/record/532017 |
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| Elgharsalli, Refka; Aloui-bejaoui, Nejla; Sala, Hedi; Chollet, Bruno; Joly, Jean-pierre; Robert, Maeva; Couraleau, Yann; Arzul, Isabelle. |
| Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010... |
| Tipo: Text |
Palavras-chave: Marteilia refringens; Molecular diagnosis; IGS; ITS; Transmission electron microscopy; Ostrea stentina. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23538/21422.pdf |
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| Damm, U.; Fourie, P.H.; Crous, P.W.. |
| Species of the genus Coniochaeta (anamorph: Lecythophora) are known as pathogens of woody hosts, but can also cause opportunistic human infections. Several fungi with conidial stages resembling Lecythophora were isolated from necrotic wood samples of Prunus trees in South Africa. In order to reveal their phylogenetic relationships, these fungi were studied on a morphological and molecular (5.8S nrDNA, ITS-1, ITS-2, GAPDH, EF-1α, 28S nrDNA, 18S nrDNA) basis. Some of the isolates were identified as Coniochaeta (Sordariomycetes), including C. velutina and two new species, C. africana and C. prunicola. The majority of the isolates, however, formed pycnidial or pseudopycnidial synanamorphs and were not closely related to Coniochaeta. According to their 28S... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Collophora; Coniochaeta; EF-1α; GAPDH; ITS; Lecythophora; LSU; Pathogenicity; Phaeomoniella; Prunus; SSU; Systematics. |
| Ano: 2010 |
URL: http://www.repository.naturalis.nl/record/531834 |
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| Benzoni, F.; Stefani, F.; Stolarski, J.; Pichon, M.; Mitta, G.; Galli, P.. |
| The phylogenetic relationships of the scleractinian genus Psammocora with the other genera traditionally included in the family Siderastreidae and some Fungiidae are assessed based on combined skeletal and molecular data. P. explanulata differs from the other examined congeneric species (P. contigua, P. digitata, P. nierstraszi, P. profundacella, P. superficialis, and P. stellata) in possessing interstomatous septa between adult corallites, costae, and in having continuous buttress-like structures joining septal faces (i.e., fulturae) which typically occur in fungiids. These characters are shared with Coscinaraea wellsi but not with the remainder of the examined siderastreids (the congeneric C. columna, and Anomastraea irregularis, Horastrea indica,... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Psammocora; Siderastreidae; Fungiidae; Synapticulae; Fulturae; ITS; Statistical parsimony; 42.79. |
| Ano: 2007 |
URL: http://www.repository.naturalis.nl/record/217424 |
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| Sosa,Daynet; Pérez Martínez,Simón; Molina,Sandy; Demey,Jhonny; Gómez,Kelly; Domínguez,Diamarys; Istúriz,María; Rumbos,Raisa; Parra,Dercy. |
| Se estudió la diversidad genética entre 68 aislados de Trichoderma procedentes de diferentes cultivos, sustratos y localidades, mediante los análisis ITS-AFLP. Se secuenció la región ITS1-5.8S-ITS2 del ADN ribosomal amplificada con los cebadores ITS1 e ITS4 y en el caso de los AFLP se utilizaron cuatro combinaciones de oligonucleótidos. Las relaciones genéticas entre los aislados se analizaron mediante el uso combinado del Análisis de Coordenadas Principales, el Análisis de Conglomerados y el ajuste de un Biplot Logístico Externo sobre datos de disimilitud, utilizando los coeficientes de Jaccard, Emparejamiento simple, Dice y Rogers y Tanimoto. Se identificaron nueve especies y las más abundantes fueron Hypocrea lixii (anamorfoTrichoderma harzianum) y T.... |
| Tipo: Journal article |
Palavras-chave: Trichoderma; ITS; AFLP; Polimorfismo; Control biológico. |
| Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000100005 |
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| LAVIN,PARIS; GÓMEZ,PATRICIA; GONZÁLE,BERNARDO; ULLOA,OSVALDO. |
| In order to assess the appropriateness of the use of internal transcribed spacer (ITS) sequences for the study of population genetics of marine cyanobacteria, we amplified and cloned the 16S rRNA gene plus the 16S-23S ITS regions of six strains of Prochlorococcus and Synechococcus. We analyzed them by denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphisms (T-RFLP). When using the standard application of these techniques, we obtained more than one band or terminal restriction fragment (T-RF) per strain or cloned sequence. Reports in literature have suggested that these anomalies can result from the formation of secondary structures. Secondary structures of the ITS sequences of Prochlorococcus and Synechococcus... |
| Tipo: Journal article |
Palavras-chave: Cyanobacteria; DGGE; T-RFLP; ITS; Secondary structure. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2008000400006 |
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| Walther, G.; Pawłowska, J.; Alastruey-Izquierdo, A.; Wrzosek, M.; Rodriguez-Tudela, J.L.; Dolatabadi, S.; Chakrabarti, A.; Hoog, G.S. de. |
| The order Mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. To inventory biodiversity in Mucorales ITS barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. Using the ITS sequences, Molecular Operational Taxonomic Units were defined by a similarity threshold of 99 %. An LSU sequence was generated for each unit as well. Analysis of the LSU sequences revealed that conventional phenotypic classifications of the Mucoraceae are highly artificial. The LSU- and ITS-based trees suggest that characters, such as rhizoids and... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Backusella; Biodiversity; Clinical relevance; DNA barcoding; Intraspecific variability; ITS; LSU; Mucor; Mucorales; Nomenclature; Rhizomucor; Taxonomy; Zygorhynchus. |
| Ano: 2013 |
URL: http://www.repository.naturalis.nl/record/532353 |
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| Ferrada,Sandra; Hernández,Klaudia; Montoya,R.; Galleguillos,Ricardo. |
| Se estudió la variabilidad de 2 genes nucleares, el intrón 4/5 del gen de Calmodulina (CaM) por amplificación PCR y la región ITS (Internal Transcribed Spacers) del ADNr, por PCR y digestión en 139 ejemplares de E. ringens provenientes de las costas de Iquique (20°13'S; 70°09'W) y Talcahuano (36°41'S; 73°06'W). Para CaM se identificaron 8 genotipos en las muestras de Talcahuano y 7 en Iquique, presentando 1 a 3 fragmentos amplificados de 550 a 1.100 pb. En la digestión del fragmento ITS amplificado, sólo la endonucleasa TaqªI detectó 3 diferentes genotipos, 2 presentes en Talcahuano y 3 en Iquique. Se discuten las frecuencias observadas para cada gen y área de estudio. Los resultados llevan a confirmar la existencia de un solo stock, desde un punto de... |
| Tipo: Journal article |
Palavras-chave: Engraulis ringens; ITS; CaM; Variabilidad genética; Stock. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382002000200022 |
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| Hershkovitz,Mark A. |
| Se determinaron las secuencias del espaciador interno (ITS) del ADN ribosomal y del espaciador intergénico de ycf3-trnS del ADN del cloroplasto para 183 muestras de taxas, chilenos y no chilenos, de Portulacaceae de América occidental, y sus grupos externos. Los datos permiten refinar previas inferencias sobre la circunscripción e interrelaciones de los géneros estudiados. En particular estos datos revelan que una circunscripción anteriormente publicada de Cistanthe Spach es polifilética y también revelan la existencia de un clado norteamericano compuesto por Claytonia L., Lewisia Pursh, Lewisiopsis R. Govaerts, and Montia L. De los datos de los géneros sudamericanos surgieron dos patrones: (1) en algunos casos la divergencia interespecífica es... |
| Tipo: Journal article |
Palavras-chave: Portulacaceae; Chile; ITS; Ycf3-trnS; Hibridización. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432006000100002 |
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| Elsalam,KA; Youssef,K; Almoammar,H. |
| Losses due to postharvest decay may occur at any time during postharvest handling, from harvest to consumption affecting the produce quality and quantity. Accurate identification of the pathogen causing postharvest disease is essential to the selection of an appropriate disease control approach. Nine isolates of Fusarium recovered from orange fruit were identified as Fusarium solani. The fungus is involved with fruit decay. The obtained cultures were purified and grown on potato-dextrose agar (PDA), malt yeast agar (MYA), and Czapek's nutrient media (CNM) under light for identification. A pathogenicity test was carried out to fulfil Koch's postulates. The pathogen could only enter ripe orange fruit through wounds and cracks causing the rot disease. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fusarium fruit rot; Orange; Pathogenicity; ITS. |
| Ano: 2015 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000100018 |
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| Registros recuperados: 69 | |
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